Review



n2b27 2i lif imaging medium  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    Thermo Fisher n2b27 2i lif imaging medium
    N2b27 2i Lif Imaging Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2i+lif/Phenol+Red/pm41896269-260-12-24
    Average 99 stars, based on 1 article reviews
    n2b27 2i lif imaging medium - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Derivative Assay:

    Article Title: Mapping and quantifying nascent transcript start sites using TT-TSS-seq.
    Article Snippet: .. Methods Yeast growth conditions S. cerevisiae BY4741 strain background (derived from S288C) were grown in liquid cultures (YPD [1.0% {w/v} yeast extract, 2.0% {w/v)}peptone, 2.0% {w/v} glucose, and supplemented with uracil {2.4 mg/L} and adenine{1.2mg/L}]) in an incubator/shaker (30°C, 300 rpm) until the exponential phase. mESC culturing mESCs (HM1) were grown in 2i/LIF (49% DMEM/F12–/–, 49% Neurobasal, 1× N2, 1× B27, 0.05 mM 2-mercaptoethanol, 2 mM L-glutamine [all of the previous from Gibco], recombinant mouse 50,000 units LIF [ESGRO], 1 μM PD03259010 [Stemgent], 3 μM CHIR 99021 [TOCRIS], and 1% penicillin/ streptomycin). ..

    Recombinant:

    Article Title: Mapping and quantifying nascent transcript start sites using TT-TSS-seq.
    Article Snippet: .. Methods Yeast growth conditions S. cerevisiae BY4741 strain background (derived from S288C) were grown in liquid cultures (YPD [1.0% {w/v} yeast extract, 2.0% {w/v)}peptone, 2.0% {w/v} glucose, and supplemented with uracil {2.4 mg/L} and adenine{1.2mg/L}]) in an incubator/shaker (30°C, 300 rpm) until the exponential phase. mESC culturing mESCs (HM1) were grown in 2i/LIF (49% DMEM/F12–/–, 49% Neurobasal, 1× N2, 1× B27, 0.05 mM 2-mercaptoethanol, 2 mM L-glutamine [all of the previous from Gibco], recombinant mouse 50,000 units LIF [ESGRO], 1 μM PD03259010 [Stemgent], 3 μM CHIR 99021 [TOCRIS], and 1% penicillin/ streptomycin). ..



    Similar Products

    2i lif media  (InvivoGen)
    98
    InvivoGen 2i lif media
    2i Lif Media, supplied by InvivoGen, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2i+lif/Hygromycin+B+Gold/pm42477113-606-7-16
    Average 98 stars, based on 1 article reviews
    2i lif media - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    n2b27 2i lif imaging medium  (Thermo Fisher)
    99
    Thermo Fisher n2b27 2i lif imaging medium
    N2b27 2i Lif Imaging Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2i+lif/Phenol+Red/pm41896269-260-12-24
    Average 99 stars, based on 1 article reviews
    n2b27 2i lif imaging medium - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    2i lif  (Tocris)
    96
    Tocris 2i lif
    2i Lif, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2i+lif/CHIR+99021/us12570957-733-17-28
    Average 96 stars, based on 1 article reviews
    2i lif - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    2i lif medium  (Biogems International)
    95
    Biogems International 2i lif medium
    a Schematic overview of three distinct culture conditions used for mouse ES cell propagation: <t>FBS–LIF,</t> <t>2i-LIF</t> and 2i-LIF-KSR. b Western blot analysis of Dnmt proteins and cofactors in R1 mouse ES cells maintained under the indicated conditions at early and late passages. Molecular weight marker sizes (kDa) are indicated on the left. c Temporal dynamics of Dnmt3L and Dnmt3A expression at the transcript (bars) and protein (lines) levels during long-term propagation in 2i-LIF (left) and 2i-LIF-KSR (right) conditions ( n = 4). Outlier values falling outside the plotted range are indicated as numbers above the corresponding passages. d Reactivation of Dnmt3L and other de novo Dnmts at the transcript and protein levels upon re-exposure to FBS–LIF after extended 2i-LIF or 2i-LIF-KSR culture ( n = 4). e Heat map illustrating the expression changes of transcripts associated with pluripotency, germline development, genomic imprinting and DNA methylation under distinct culture conditions. Expression values were calculated from four independent biological replicates. f CHX-chase assay to assess the protein stabilities of Dnmt3L and Dnmt3A2 in mouse ES cells at p6 under the 2i-LIF condition. Short (s.e) and long (l.e) exposures are shown. g Quantitative analysis of Dnmt3L (red), Dnmt3A2 (black) and Dnmt3B (blue) protein levels from the CHX-chase assay ( n = 4). Protein expression was normalized to β-actin. Quantitative data are represented as mean ± standard error of the mean (s.e.m.). Statistical analysis was performed using a two-way ( c and g ) or one-way ( d ) ANOVA with the Bonferroni post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with control groups. ### P < 0.001 compared with Dnmt3A2. Exact P values and replicate numbers are provided in the . See also Supplementary Figs. and .
    2i Lif Medium, supplied by Biogems International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2i+lif/CHIR+99021/pmc13049025-43-10-16
    Average 95 stars, based on 1 article reviews
    2i lif medium - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    2i lif media  (Biosynth Carbosynth)
    93
    Biosynth Carbosynth 2i lif media
    a Schematic overview of three distinct culture conditions used for mouse ES cell propagation: <t>FBS–LIF,</t> <t>2i-LIF</t> and 2i-LIF-KSR. b Western blot analysis of Dnmt proteins and cofactors in R1 mouse ES cells maintained under the indicated conditions at early and late passages. Molecular weight marker sizes (kDa) are indicated on the left. c Temporal dynamics of Dnmt3L and Dnmt3A expression at the transcript (bars) and protein (lines) levels during long-term propagation in 2i-LIF (left) and 2i-LIF-KSR (right) conditions ( n = 4). Outlier values falling outside the plotted range are indicated as numbers above the corresponding passages. d Reactivation of Dnmt3L and other de novo Dnmts at the transcript and protein levels upon re-exposure to FBS–LIF after extended 2i-LIF or 2i-LIF-KSR culture ( n = 4). e Heat map illustrating the expression changes of transcripts associated with pluripotency, germline development, genomic imprinting and DNA methylation under distinct culture conditions. Expression values were calculated from four independent biological replicates. f CHX-chase assay to assess the protein stabilities of Dnmt3L and Dnmt3A2 in mouse ES cells at p6 under the 2i-LIF condition. Short (s.e) and long (l.e) exposures are shown. g Quantitative analysis of Dnmt3L (red), Dnmt3A2 (black) and Dnmt3B (blue) protein levels from the CHX-chase assay ( n = 4). Protein expression was normalized to β-actin. Quantitative data are represented as mean ± standard error of the mean (s.e.m.). Statistical analysis was performed using a two-way ( c and g ) or one-way ( d ) ANOVA with the Bonferroni post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with control groups. ### P < 0.001 compared with Dnmt3A2. Exact P values and replicate numbers are provided in the . See also Supplementary Figs. and .
    2i Lif Media, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2i+lif/4-Thiouridine/bio_rxiv__64898__2026__02__26__708215-350-7-15
    Average 93 stars, based on 1 article reviews
    2i lif media - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    2i lif  (Thermo Fisher)
    99
    Thermo Fisher 2i lif
    a Schematic overview of three distinct culture conditions used for mouse ES cell propagation: <t>FBS–LIF,</t> <t>2i-LIF</t> and 2i-LIF-KSR. b Western blot analysis of Dnmt proteins and cofactors in R1 mouse ES cells maintained under the indicated conditions at early and late passages. Molecular weight marker sizes (kDa) are indicated on the left. c Temporal dynamics of Dnmt3L and Dnmt3A expression at the transcript (bars) and protein (lines) levels during long-term propagation in 2i-LIF (left) and 2i-LIF-KSR (right) conditions ( n = 4). Outlier values falling outside the plotted range are indicated as numbers above the corresponding passages. d Reactivation of Dnmt3L and other de novo Dnmts at the transcript and protein levels upon re-exposure to FBS–LIF after extended 2i-LIF or 2i-LIF-KSR culture ( n = 4). e Heat map illustrating the expression changes of transcripts associated with pluripotency, germline development, genomic imprinting and DNA methylation under distinct culture conditions. Expression values were calculated from four independent biological replicates. f CHX-chase assay to assess the protein stabilities of Dnmt3L and Dnmt3A2 in mouse ES cells at p6 under the 2i-LIF condition. Short (s.e) and long (l.e) exposures are shown. g Quantitative analysis of Dnmt3L (red), Dnmt3A2 (black) and Dnmt3B (blue) protein levels from the CHX-chase assay ( n = 4). Protein expression was normalized to β-actin. Quantitative data are represented as mean ± standard error of the mean (s.e.m.). Statistical analysis was performed using a two-way ( c and g ) or one-way ( d ) ANOVA with the Bonferroni post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with control groups. ### P < 0.001 compared with Dnmt3A2. Exact P values and replicate numbers are provided in the . See also Supplementary Figs. and .
    2i Lif, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2i+lif/2+MERCAPTOETHANOL/pm41702708-163-52-72
    Average 99 stars, based on 1 article reviews
    2i lif - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier
    2i lif medium  (Tocris)
    96
    Tocris 2i lif medium
    a Schematic overview of three distinct culture conditions used for mouse ES cell propagation: <t>FBS–LIF,</t> <t>2i-LIF</t> and 2i-LIF-KSR. b Western blot analysis of Dnmt proteins and cofactors in R1 mouse ES cells maintained under the indicated conditions at early and late passages. Molecular weight marker sizes (kDa) are indicated on the left. c Temporal dynamics of Dnmt3L and Dnmt3A expression at the transcript (bars) and protein (lines) levels during long-term propagation in 2i-LIF (left) and 2i-LIF-KSR (right) conditions ( n = 4). Outlier values falling outside the plotted range are indicated as numbers above the corresponding passages. d Reactivation of Dnmt3L and other de novo Dnmts at the transcript and protein levels upon re-exposure to FBS–LIF after extended 2i-LIF or 2i-LIF-KSR culture ( n = 4). e Heat map illustrating the expression changes of transcripts associated with pluripotency, germline development, genomic imprinting and DNA methylation under distinct culture conditions. Expression values were calculated from four independent biological replicates. f CHX-chase assay to assess the protein stabilities of Dnmt3L and Dnmt3A2 in mouse ES cells at p6 under the 2i-LIF condition. Short (s.e) and long (l.e) exposures are shown. g Quantitative analysis of Dnmt3L (red), Dnmt3A2 (black) and Dnmt3B (blue) protein levels from the CHX-chase assay ( n = 4). Protein expression was normalized to β-actin. Quantitative data are represented as mean ± standard error of the mean (s.e.m.). Statistical analysis was performed using a two-way ( c and g ) or one-way ( d ) ANOVA with the Bonferroni post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with control groups. ### P < 0.001 compared with Dnmt3A2. Exact P values and replicate numbers are provided in the . See also Supplementary Figs. and .
    2i Lif Medium, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2i+lif/PD+0325901/pm41219433-330-10-19
    Average 96 stars, based on 1 article reviews
    2i lif medium - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    2i lif medium  (TaKaRa)
    96
    TaKaRa 2i lif medium
    a Schematic overview of three distinct culture conditions used for mouse ES cell propagation: <t>FBS–LIF,</t> <t>2i-LIF</t> and 2i-LIF-KSR. b Western blot analysis of Dnmt proteins and cofactors in R1 mouse ES cells maintained under the indicated conditions at early and late passages. Molecular weight marker sizes (kDa) are indicated on the left. c Temporal dynamics of Dnmt3L and Dnmt3A expression at the transcript (bars) and protein (lines) levels during long-term propagation in 2i-LIF (left) and 2i-LIF-KSR (right) conditions ( n = 4). Outlier values falling outside the plotted range are indicated as numbers above the corresponding passages. d Reactivation of Dnmt3L and other de novo Dnmts at the transcript and protein levels upon re-exposure to FBS–LIF after extended 2i-LIF or 2i-LIF-KSR culture ( n = 4). e Heat map illustrating the expression changes of transcripts associated with pluripotency, germline development, genomic imprinting and DNA methylation under distinct culture conditions. Expression values were calculated from four independent biological replicates. f CHX-chase assay to assess the protein stabilities of Dnmt3L and Dnmt3A2 in mouse ES cells at p6 under the 2i-LIF condition. Short (s.e) and long (l.e) exposures are shown. g Quantitative analysis of Dnmt3L (red), Dnmt3A2 (black) and Dnmt3B (blue) protein levels from the CHX-chase assay ( n = 4). Protein expression was normalized to β-actin. Quantitative data are represented as mean ± standard error of the mean (s.e.m.). Statistical analysis was performed using a two-way ( c and g ) or one-way ( d ) ANOVA with the Bonferroni post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with control groups. ### P < 0.001 compared with Dnmt3A2. Exact P values and replicate numbers are provided in the . See also Supplementary Figs. and .
    2i Lif Medium, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2i+lif/NDiff+227/pmc12543326-138-0-8
    Average 96 stars, based on 1 article reviews
    2i lif medium - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    2i lif medium  (Selleck Chemicals)
    96
    Selleck Chemicals 2i lif medium
    a Schematic overview of three distinct culture conditions used for mouse ES cell propagation: <t>FBS–LIF,</t> <t>2i-LIF</t> and 2i-LIF-KSR. b Western blot analysis of Dnmt proteins and cofactors in R1 mouse ES cells maintained under the indicated conditions at early and late passages. Molecular weight marker sizes (kDa) are indicated on the left. c Temporal dynamics of Dnmt3L and Dnmt3A expression at the transcript (bars) and protein (lines) levels during long-term propagation in 2i-LIF (left) and 2i-LIF-KSR (right) conditions ( n = 4). Outlier values falling outside the plotted range are indicated as numbers above the corresponding passages. d Reactivation of Dnmt3L and other de novo Dnmts at the transcript and protein levels upon re-exposure to FBS–LIF after extended 2i-LIF or 2i-LIF-KSR culture ( n = 4). e Heat map illustrating the expression changes of transcripts associated with pluripotency, germline development, genomic imprinting and DNA methylation under distinct culture conditions. Expression values were calculated from four independent biological replicates. f CHX-chase assay to assess the protein stabilities of Dnmt3L and Dnmt3A2 in mouse ES cells at p6 under the 2i-LIF condition. Short (s.e) and long (l.e) exposures are shown. g Quantitative analysis of Dnmt3L (red), Dnmt3A2 (black) and Dnmt3B (blue) protein levels from the CHX-chase assay ( n = 4). Protein expression was normalized to β-actin. Quantitative data are represented as mean ± standard error of the mean (s.e.m.). Statistical analysis was performed using a two-way ( c and g ) or one-way ( d ) ANOVA with the Bonferroni post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with control groups. ### P < 0.001 compared with Dnmt3A2. Exact P values and replicate numbers are provided in the . See also Supplementary Figs. and .
    2i Lif Medium, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2i+lif/CHIR-99021/pm40696106-376-43-53
    Average 96 stars, based on 1 article reviews
    2i lif medium - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    a Schematic overview of three distinct culture conditions used for mouse ES cell propagation: FBS–LIF, 2i-LIF and 2i-LIF-KSR. b Western blot analysis of Dnmt proteins and cofactors in R1 mouse ES cells maintained under the indicated conditions at early and late passages. Molecular weight marker sizes (kDa) are indicated on the left. c Temporal dynamics of Dnmt3L and Dnmt3A expression at the transcript (bars) and protein (lines) levels during long-term propagation in 2i-LIF (left) and 2i-LIF-KSR (right) conditions ( n = 4). Outlier values falling outside the plotted range are indicated as numbers above the corresponding passages. d Reactivation of Dnmt3L and other de novo Dnmts at the transcript and protein levels upon re-exposure to FBS–LIF after extended 2i-LIF or 2i-LIF-KSR culture ( n = 4). e Heat map illustrating the expression changes of transcripts associated with pluripotency, germline development, genomic imprinting and DNA methylation under distinct culture conditions. Expression values were calculated from four independent biological replicates. f CHX-chase assay to assess the protein stabilities of Dnmt3L and Dnmt3A2 in mouse ES cells at p6 under the 2i-LIF condition. Short (s.e) and long (l.e) exposures are shown. g Quantitative analysis of Dnmt3L (red), Dnmt3A2 (black) and Dnmt3B (blue) protein levels from the CHX-chase assay ( n = 4). Protein expression was normalized to β-actin. Quantitative data are represented as mean ± standard error of the mean (s.e.m.). Statistical analysis was performed using a two-way ( c and g ) or one-way ( d ) ANOVA with the Bonferroni post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with control groups. ### P < 0.001 compared with Dnmt3A2. Exact P values and replicate numbers are provided in the . See also Supplementary Figs. and .

    Journal: Experimental & Molecular Medicine

    Article Title: Uncovering the acetylation sites of Dnmt3L that regulate protein stability and differentiation potency in embryonic stem cells

    doi: 10.1038/s12276-026-01655-w

    Figure Lengend Snippet: a Schematic overview of three distinct culture conditions used for mouse ES cell propagation: FBS–LIF, 2i-LIF and 2i-LIF-KSR. b Western blot analysis of Dnmt proteins and cofactors in R1 mouse ES cells maintained under the indicated conditions at early and late passages. Molecular weight marker sizes (kDa) are indicated on the left. c Temporal dynamics of Dnmt3L and Dnmt3A expression at the transcript (bars) and protein (lines) levels during long-term propagation in 2i-LIF (left) and 2i-LIF-KSR (right) conditions ( n = 4). Outlier values falling outside the plotted range are indicated as numbers above the corresponding passages. d Reactivation of Dnmt3L and other de novo Dnmts at the transcript and protein levels upon re-exposure to FBS–LIF after extended 2i-LIF or 2i-LIF-KSR culture ( n = 4). e Heat map illustrating the expression changes of transcripts associated with pluripotency, germline development, genomic imprinting and DNA methylation under distinct culture conditions. Expression values were calculated from four independent biological replicates. f CHX-chase assay to assess the protein stabilities of Dnmt3L and Dnmt3A2 in mouse ES cells at p6 under the 2i-LIF condition. Short (s.e) and long (l.e) exposures are shown. g Quantitative analysis of Dnmt3L (red), Dnmt3A2 (black) and Dnmt3B (blue) protein levels from the CHX-chase assay ( n = 4). Protein expression was normalized to β-actin. Quantitative data are represented as mean ± standard error of the mean (s.e.m.). Statistical analysis was performed using a two-way ( c and g ) or one-way ( d ) ANOVA with the Bonferroni post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001 compared with control groups. ### P < 0.001 compared with Dnmt3A2. Exact P values and replicate numbers are provided in the . See also Supplementary Figs. and .

    Article Snippet: To maintain naive pluripotency, mouse ES cells were maintained in 2i-LIF medium containing 3 μM CHIR99021 (BioGems) and 1 μM PD0325901 (BioGems) under the following two conditions on a 0.1% gelatin-coated tissue culture dish: FBS free or supplemented with knockout serum replacement (KSR) (Thermo Fisher Scientific).

    Techniques: Western Blot, Molecular Weight, Marker, Expressing, DNA Methylation Assay, Control